Additional Selective and Differential Cell Culture Media
NEOGEN Violet Red Bile Agar with MUG (VRBA-MUG), 10 kg
Violet Red Bile Agar with MUG is used for the detection of coliforms and the fluorogenic detection of Escherichia coli. Violet Red Bile Agar with MUG is not intended for use in the diagnosis of disease or other conditions in humans. Violet Red Bile Agar W/ MUG is specified in many procedures to enumerate coliforms in food and dairy products. In 1982, Feng and Hartman developed a rapid fluorogenic assay for Escherichia coli by incorporating 4-methylumbelliferyl-β-D-glucuronide (MUG) into Lauryl Tryptose Broth. Similarly, incorporating MUG into Violet Red Bile Agar permits the detection of E. coli among the coliform colonies. Standard methods procedures specify Violet Red Bile Agar with MUG for detecting E. coli in food and dairy products by fluorescence. This medium is often abbreviated as VRBA w/ MUG.For more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-agar-with-mug
NEOGEN Fraser Broth, 500 g
Fraser Broth is used with ferric ammonium citrate for the selective enrichment of Listeria species and is not intended for use in the diagnosis of disease or other conditions in humans.Fraser Broth is a secondary enrichment broth for the isolation of Listeria spp. formulated according to ISO 11290. The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate (X211 or 7984) is added to the tempered broth after sterilization.Formula:Peptone - 15.0 g/LYeast Extract - 5.0 g/LEsculin - 1.0 g/LDisodium Hydrogen Phosphate - 9.6 g/LPotassium Dihydrogen Phosphate - 1.35 g/LSodium Chloride - 20.0 g/LLithium Chloride - 3.0 g/LAcriflavine - 0.025 g/LNalidixic Acid - 0.02 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/fraser-broth
NEOGEN Fraser Broth Base, 500 g
Fraser Broth Base is used with Acriflavin, Nalidixic Acid, and Ferric Ammonium Citrate for the selective enrichment of Listeria spp. Fraser Broth Base is not intended for use in the diagnosis of disease or other conditions in humans.Formulated according to MLG Appendix 1.09 (for the ISO 11290 formulation please use NCM0001). Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis indicated the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese and pate.For more information visit https://www.neogen.com/solutions/Microbiology/fraser-broth-base
NEOGEN Orange Serum Agar, 500 g
Orange Serum Agar is used for the cultivation of aciduric microorganisms associated with spoilage of products and is not intended for use in the diagnosis of disease or other conditions in humans.The low pH of fruit juices makes citrus fruit products susceptible to spoilage by yeasts, molds, and the bacteria Lactobacillus and Leuconostoc. In the 1950’s, Hays investigated spoilage in frozen concentrated orange juice. He found that an agar medium containing orange serum (juice) was superior to Lindegren Agar in isolating the microorganisms responsible for spoilage causing a buttermilk off-odor. Murdock, Folinazzo, and Troy found Orange Serum Agar, pH 5.4 to be a suitable medium for growing Leuconostoc, Lactobacillus, and yeasts. Orange Serum Agar is recommended for examining fruit beverages.For more information visit https://www.neogen.com/solutions/Microbiology/orange-serum-agar
NEOGEN EC Broth, 10 kg
EC Broth is used for the detection of coliform bacteria at 37°C and Escherichia coli at 44.5°C. EC Broth is not intended for use in the diagnosis of disease or other conditions in humans.EC Broth (Escherichia coli Broth) is a selective enrichment broth designed for the isolation of coliforms, including E. coli, from water and food samples. It was the recommended medium of the American Public Health Association (APHA) and the AOAC.EC Broth is made selective for coliforms by the inclusion of Bile Salts in the dehydrated medium. The selective nature of this medium ensures that the growth of non-coliform bacteria is minimized. The medium is buffered by the addition of potassium phosphates and osmotically balanced by sodium chloride. The medium is used at 37°C for coliform organisms and 44.5°C is recommended for the isolation E. coli.For more information visit https://www.neogen.com/solutions/Microbiology/ec-broth
NEOGEN D/E Neutralizing Broth with Tween, 500 g
D/E Neutralizing Broth is used to neutralize and determine the bactericidal activity of antiseptics and disinfectants. D/E Neutralizing Broth is not intended for use in the diagnosis of disease or other conditions in humans.D/E Neutralizing Broth was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycollate media, and Neutralizing Buffer.For more information visit https://www.neogen.com/solutions/Microbiology/de-neutralizing-broth-with-tween
NEOGEN Salmonella Shigella (SS) Agar, 500 g
Salmonella Shigella (SS) Agar is used for the isolation of Salmonella spp. and some strains of Shigella spp. Salmonella Shigella (SS) Agar is not intended for use in the diagnosis of disease or other conditions in humans. Salmonella Shigella (SS) Agar is a modification of the Desoxycholate Citrate Agar described by Leifson. Salmonella Shigella Agar is superior to a number of other media for the isolation of Salmonella spp. and Shigella spp. Salmonella Shigella (SS) Agar is recommended for testing food samples for the presence of Salmonella spp. and some Shigella spp. Formula:Beef Extract - 5.0 g/LEnzymatic Digest of Casein - 2.5 g/LEnzymatic Digest of Animal Tissue - 2.5 g/LLactose - 10.0 g/LBile Salts - 8.5 g/LSodium Citrate - 8.5 g/LSodium Thiosulfate - 8.5 g/LFerric Citrate - 1.0 g/LBrilliant Green - 0.00033 g/LNeutral Red - 0.025 g/L Agar - 13.5 g/L For more information visit https://www.neogen.com/solutions/Microbiology/salmonella-shigella-agar
NEOGEN YM Broth, 5 kg
YM Broth is used for the cultivation of fungi in a laboratory setting. YM Broth is not intended for use in the diagnosis of disease or other conditions in humans. YM Broth is prepared according to the formulation published by Wickerham. Wickerham suggested that YM Broth, acidified to pH 3.0 – 4.0, be used as an enrichment medium for yeasts from populations also containing bacteria and molds. To favor isolation of fermentative species, add a layer of sterile paraffin oil 1 cm deep on the surface of the inoculated broth. Cultures should be incubated until growth appears, and then streaked onto YM Agar to obtain isolated yeast colonies. To isolate fermentative and oxidative strains, place acidified inoculated YM Broth on a rotary shaker for 1 to 2 days. This favors yeast recovery while preventing sporulation of molds. Media selectivity may be enhanced through acidification or addition of selective agents.For more information visit https://www.neogen.com/solutions/Microbiology/ym-broth
NEOGEN Sabouraud Dextrose Agar with Chloramphenicol, 500 g
Sabouraud Dextrose Agar with Chloramphenicol is used for the selective isolation of fungi in a laboratory setting. Sabouraud Dextrose Agar with Chloramphenicol is not intended for use in the diagnosis of disease or other conditions in humans. Sabouraud Dextrose Agar (SDA) is a modification of Dextrose Agar described by Sabouraud. Sabouraud Dextrose Agar is used for cultivating pathogenic and commensal fungi and yeasts. The high dextrose concentration and acidic pH of the formula permits selectivity of fungi. This medium is beneficial in sporulation studies and pigment production. Sabouraud Dextrose Agar is used for determining the microbial content of cosmetics and in the mycological evaluation of food.For more information visit https://www.neogen.com/solutions/Microbiology/sabouraud-dextrose-agar-with-chloramphenicol
NEOGEN Sabouraud Dextrose Agar with Chloramphenicol, 10 kg
Sabouraud Dextrose Agar with Chloramphenicol is used for the selective isolation of fungi in a laboratory setting. Sabouraud Dextrose Agar with Chloramphenicol is not intended for use in the diagnosis of disease or other conditions in humans. Sabouraud Dextrose Agar (SDA) is a modification of Dextrose Agar described by Sabouraud. Sabouraud Dextrose Agar is used for cultivating pathogenic and commensal fungi and yeasts. The high dextrose concentration and acidic pH of the formula permits selectivity of fungi. This medium is beneficial in sporulation studies and pigment production. Sabouraud Dextrose Agar is used for determining the microbial content of cosmetics and in the mycological evaluation of food.For more information visit https://www.neogen.com/solutions/Microbiology/sabouraud-dextrose-agar-with-chloramphenicol
NEOGEN Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar, 10 kg
Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios according to ISO 21872-1:2017 and ISO 21872-2:2007. Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is not intended for use in the diagnosis of disease or other conditions in humans. TCBS Agar is also called Vibrio Selective Agar, and prepared according to the formula of Kobayashi et al. This formula is a modification of the selective medium from Nakanishi. All pathogenic Vibrio spp., except Vibrio hollisae, will grow on TCBS Agar. This highly selective agar meets the nutritional requirements of Vibrio spp. Vibrio spp. are able to grow in media containing increased salt concentrations, and some species are halophilic.For more information visit https://www.neogen.com/solutions/Microbiology/thiosulfate-citrate-bile-salts-sucrose-agar
NEOGEN Buffered Listeria Enrichment Broth, 500 g
Buffered Listeria Enrichment Broth is used for selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. Evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-listeria-enrichment-broth
NEOGEN Terrific Broth, Modified, 500 g
Terrific Broth, Modified is used with glycerol in cultivating recombinant strains of Escherichia coli in a laboratory setting. Terrific Broth, Modified is not intended for use in the diagnosis of disease or other conditions in humans. Terrific Broth, Modified was developed by Tartoff and Hobbs to increase yields of plasmid DNA from transformed E. coli and is a popular choice of media available for plasmid preps. While cells grown in Terrific Broth, Modified can grow slower than other liquid media, the final cell density and plasmid yields are 2 – 4 fold higher. The higher yield is a result of the increased concentration of Enzymatic Digest of Casein and Yeast Extract in this formula. Glycerol is used as a carbohydrate source, and unlike glucose, is not fermented to acetic acid.For more information visit https://www.neogen.com/solutions/Microbiology/terrific-broth-modified
NEOGEN Oxbile (Oxgall), 500 g
Oxbile (Oxgall) is dehydrated bile for use in preparing microbiological culture media in a laboratory setting. Oxbile (Oxgall) is not intended for use in the diagnosis of disease or other conditions in humans. Oxbile is manufactured from large quantities of fresh bile by rapid evaporation of the water content. Bile is composed of fatty acids, bile acids, inorganic salts, sulfates, bile pigments, cholesterol, mucin, lecithin, glycuronicacids, porphyrins, and urea. The use of Oxbile insures a regular supply of bile, and uniformity impossible to obtain with fresh materials. Oxbile is dehydrated fresh bile and prepared specifically for differentiation of bile tolerant microorganisms. A 10% solution of dehydrated bile is equivalent to a fresh bile solution. It is usually incorporated into media e.g., Bile Esculin Agar and Brilliant Green Bile Agar, used for the determination of enteric pathogens.For more information visit https://www.neogen.com/solutions/Microbiology/oxbile-oxgall
NEOGEN Acuferm Neopeptone, 10 kg
For more information visit https://www.neogen.com/solutions/Microbiology/acuferm-neopeptone